Adaptation of Madin-Darby canine kidney cells to hypertonic medium: An electron microprobe analysis
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منابع مشابه
Sorting of sphingolipids in epithelial (Madin-Darby canine kidney) cells
To study the intracellular transport of newly synthesized sphingolipids in epithelial cells we have used a fluorescent ceramide analog, N-6[7-nitro-2,1,3-benzoxadiazol-4-yl] aminocaproyl sphingosine (C6-NBD-ceramide; Lipsky, N. G., and R. E. Pagano, 1983, Proc. Natl. Acad. Sci. USA, 80:2608-2612) as a probe. This ceramide was readily taken up by filter-grown Madin-Darby canine kidney (MDCK) cel...
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The Madin-Darby canine kidney (MDCK) cell line, derived from distal tubule/ collecting duct, expresses differentiated properties of renal tubule epithelium in culture. We studied the expression of adrenergic receptors in MDCK to examine the role of catecholamines in the regulation of renal function. Radioligand-binding studies demonstrated, on the basis of receptor affinities of subtype-selecti...
متن کاملEndocytosis in filter-grown Madin-Darby canine kidney cells
In this paper, we have characterized the apical and basolateral endocytic pathways of epithelial MDCK cells grown on filters. The three-dimensional organization of the endocytic compartments was analyzed by confocal microscopy after internalization of a fluorescent fluid-phase marker from either side of the cell layer. After 5 min of internalization, distinct sets of apical and basolateral earl...
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13 annexins have so far been identified in mammals, but members of the family are spread out all over the eukaryotic kingdom (Morgan and Fernandez, 1997). Each annexin has a distinct N terminus and annexins are homologous in the Cterminal core, a domain of 30 kDa that harbors calcium binding sites (Liemann and Huber, 1997). In vitro binding experiments between purified annexins and liposomes ha...
متن کاملTitle: Ca 2+ movement and apoptosis induced by deltamethrin in Madin – Darby canine kidney canine renal tubular cells Keywords: Apoptosis, Ca2+, Deltamethrin, Madin–Darby canine kidney, Renal cells
Title: Ca 2+ movement and apoptosis induced by deltamethrin in Madin – Darby canine kidney canine renal tubular cells Keywords: Alveolar bone grafting, Antigen-extracted xenogeneic cancellous bone, Osteogenesis, Recombinant human bone morphogenetic protein-2 26-33 Title: Clinical performance of a new hepatitis B surface antigen quantitative assay with automatic dilution Title: Noncontrast compu...
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ژورنال
عنوان ژورنال: Kidney International
سال: 1998
ISSN: 0085-2538
DOI: 10.1046/j.1523-1755.1998.06725.x